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Mixed Culture of Aerobic and Anaerobic Microorganisms Under Similar Condition

M. C. Kim, W. S. Kim and H. G. Ryang

Institute of Plant Physiology, State Academy of Science of DPR Korea, Pyongyang, DPR Korea

Full Paper (PDF File: 314KB)

Abstract

To clear the characteristics of EM culture, mixed culture of photosynthetic bacteria, lactic acid bacteria, yeast and N₂-fixing bacteria was tested in the same medium. Rhodopseudomonas sp., Lactrobacillus sp., Saccharomyces cerevisiae and Azotobacter chroococcum were able to live together in 5% molasses medium under a certain intensity of light. The microbic equilibrium was reached on the 36^th day after culture, At this time, the number of these 4 strains were 3 x 10⁷, 5 x 10⁶, 2 x 10⁷ and 2 x 10⁶ ml, respectively and pH of culture was 6.5, Although this microbic equilibrium was broken down by changing pH to 3, 5, 7 and 9 artificially, a new microbic equilibrium was recovered again only under lighting and when the pH of the culture was 6.5. However, pH decreased under dark and the microflora changed remarkably.